Jun Antikörper

Antigen: Jun

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN131235

Produktbeschreibung

Isotyp: IgG, Ig Fraction  (Passende Sekundärantikörper)

Beschreibung: Extracellular stimuli such as growth factors and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation at Ser63/73 renders c-Jun non-responsive to mitogenic and stress induced signaling pathways. JNK (c-jun N-terminal Kinase) binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activate c-Jun.

Spezifität: Recognizes c-Jun in samples of human, mouse, and rat origin. The antibody only recognizes c-Jun, and will not detect the endogenous levels of Jun B or Jun D.

Anwendungen

Applikationshinweise: The antibody can be used for Western blot analysis (1 µg/ml) and immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually.

Buffer: Supplied as 100 µg (0.5 mg/ml) affinity purified rabbit anti-c-Jun polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Binetruy, Smeal, Karin: "Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain." in: Nature, Vol. 351, Issue 6322, pp. 122-7, 1991 (PubMed).

Derijard, Hibi, Wu et al.: "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain." in: Cell, Vol. 76, Issue 6, pp. 1025-37, 1994 (PubMed).