D4-GDI Antikörper

Antigen: D4-GDI

Synonyme: ARHGDIB

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN130959

Produktbeschreibung

Isotyp: IgG, Ig Fraction  (Passende Sekundärantikörper)

Beschreibung: D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kDa and 5kDa size fragment. The 23kDa fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Spezifität: The antibody recognizes 28 kDa D4-GDI in human samples. Not tested in other species.

Anwendungen

Applikationshinweise: The antibody can be used for Western blotting (1-2 µg/ml). However, the optimal conditions should be determined individually.

Buffer: Supplied as 100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

Beschränkungen: Nur für Forschungszwecke einsetzbar