Desmoglein 3 (DSG3) Antikörper

Antigen: Desmoglein 3 (DSG3)

Synonyme: DSG3, PVA, CDHF6, DKFZp686P23184, bal, balding

Klonalität: Monoklonal (Aic-2)

Wirt: Maus

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (IHC), Immunzytochemie (ICC)

Produktnummer: ABIN130749

Produktbeschreibung

Weitere Bezeichnung: Desmoglein-3

Immunogen: This antibody was purified from hybridoma (clone AK23) supernatant using protein A agarose. This hybridoma was established by fusion of splenocytes from PV model mouse and P3 mouse myeloma cells.

Format: Lyophilized

Isotyp: IgG1, IgM, IgG  (Passende Sekundärantikörper)

Klon: Aic-2

Beschreibung: Pemphigus vulgaris (PV) is a life -threatening autoimmune blistering disease of the skin and mucous membranes. In patients of PV, IgG autoantibodies against the cell surface of keratinocytes in stratified squamous epithelia play a major pathogenic role in loss of cell-cell adhesion. The autoimmune target of PV is desmoglein 3 (Dsg3) which is a member of the cadherin family of calcium-depending, transmembrane glycoprotein.

Spezifität: This antibody reacts with mouse and human Dsg3.

Anwendungen

Applikationshinweise: Western blotting: Not recommended. Immunoprecipitation: Clone AK23 is used in reference 1). Immunohistochemistry: 0.1 µg/mL. Immunocytochemistry: 0.1 µg/mL

Konzentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. No preservative is contained.

Forschungsgebiet: Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical detection of Dsg3 on frozen section of normal human skin with ABIN130749.

Immunohistochemical detection of Dsg3 on frozen section of mouse oral mucosa with ABIN130749.

Immunocytochemical detection of Dsg3 on living mouse keratinocyte cell line (PAM212 cells) with ABIN130749.

Publikationen

Publikationen: Tsunoda, Ota, Aoki et al.: "Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 2170-8, 2003 (PubMed).