Desmoglein 3 (DSG3) Antikörper

Antigen: Desmoglein 3 (DSG3)

Synonyme: DSG3, PVA, CDHF6, DKFZp686P23184, bal, balding

Klonalität: Monoklonal (Aic-2)

Wirt: Maus

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (IHC), Immunzytochemie (ICC)

Produktnummer: ABIN130747

Produktbeschreibung

Weitere Bezeichnung: Desmoglein-3

Immunogen: This antibody was purified from hybridoma (clone AK9) supernatant using protein A agarose. This hybridoma was established by fusion of splenocytes from PV model mouse and P3 mouse myeloma cells.

Format: Lyophilized

Isotyp: IgG1, IgM, IgG  (Passende Sekundärantikörper)

Klon: Aic-2

Beschreibung: Pemphigus vulgaris (PV) is a life -threatening autoimmune blistering disease of the skin and mucous membranes. In patients of PV, IgG autoantibodies against the cell surface of keratinocytes in stratified squamous epithelia play a major pathogenic role in loss of cell-cell adhesion. The autoimmune target of PV is desmoglein 3 (Dsg3), which is a member of the cadherin family of calcium-depending, transmembrane glycoproteins.

Spezifität: This antibody reacts with mouse Dsg3.

Anwendungen

Applikationshinweise: Western blotting: Not tested. Immunoprecipitation: Clone AK9 is used in reference 1). Immunohistochemistry: 0.01 µg/mL. Immunocytochemistry: 0.01 µg/mL. Flow Cytometry: Not tested.

Konzentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives.

Forschungsgebiet: Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical detection of mouse Dsg3 on frozen section of mouse oral mucosa with ABIN130747.

Immunocytochemical detection of Dsg3 on living mouse keratinocyte cell line (PAM212 cells) with ABIN130747.

Publikationen

Publikationen: Tsunoda, Ota, Aoki et al.: "Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 2170-8, 2003 (PubMed).