Abl-Interactor 1 (ABI1) Antikörper

Antigen: Abl-Interactor 1 (ABI1)

Synonyme: E3B1, ABI-1, NAP1BP, SSH3BP, SSH3BP1, NAP1, MGC6064, Ssh3bp1, E3b1, abi1, MGC73174, zgc:73174, wu:fc66g09, wu:fk56a10, ABI1, MGC84356, e3b1, abi-1, nap1bp, ssh3bp, ssh3bp1, MGC79488, MGC139877, abi-2, abi2b, aip-1, ablbp3, argbpia, argbpib, ssh3bp2, MGC153534, wu:fe48a10, zgc:153534, DKFZp469P1319

Klonalität: Monoklonal (1G9)

Wirt: Maus

Reaktivität: Human, Maus, Hamster

Applikation: Western Blot (WB), Immunpräzipitation (IP), Immunzytochemie (ICC), Immunhistochemie (IHC)

Produktnummer: ABIN130666

Produktbeschreibung

Weitere Bezeichnung: Abi1 (Abl-Interactor-1)/E3B1 

Immunogen: This antibody was purified from hybridoma (clone 1G9) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp/O with Balb/c mouse splenocyte immunized with a human recombinant Abi-1 expressed in E. coli.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 1G9

Beschreibung: Abl interactor (Abi) proteins are c-Abl-binding proteins that bind to both the Src homology 3 (SH3) domain and the C-terminal proline-rich regions of Abl kinase through dual SH3-PXXP interactions. Two highly related genes, abi-1 and abi-2, were cloned. In addition to the interaction with Abl kinase, Abi proteins also interact with other signaling molecules. Recently, it has been reported that Abi-1 dramatically promoted c-Abl-mediated tyrosine (Tyr 296 ) phosphorylation of Mena [mammalian homologue of Drosophila Enabled (Ena)] by interacting with both proteins.

Spezifität: This antibody reacts with Abi-1 (65 kDa) on Western blotting.

Synonyme: E3B1, ABI-1, NAP1BP, SSH3BP, SSH3BP1, NAP1, MGC6064, Ssh3bp1, E3b1, abi1, MGC73174, zgc:73174, wu:fc66g09, wu:fk56a10, ABI1, MGC84356, e3b1, abi-1, nap1bp, ssh3bp, ssh3bp1, MGC79488, MGC139877, abi-2, abi2b, aip-1, ablbp3, argbpia, argbpib, ssh3bp2, MGC153534, wu:fe48a10, zgc:153534, DKFZp469P1319

Anwendungen

Applikationshinweise: Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 200 µL of cell extract from 5x10 6 cells. Immunohistochemistry: 5 µg / mL Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5). Immunocytochemistry: Not tested. This antibody was used in immunocytochemistry in ref. 1.

Konzentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of Abi-1 expression in HeLa cells (1), 293T cells (2), Raji cells (3), Jurkat cells (4), A431 cells (5), U251 cells (6), KG1 cells (7), NIH/3T3 cells (8), WR19L cells (9) and CHO cells (10) using ABIN130666

Immunohistochemical detection of Abi - 1 on paraffin embedded section of a human lung cancer with ABIN130666.

Publikationen

Publikationen: Yang, Ta, Pessah et al.: "Functional defects in six ryanodine receptor isoform-1 (RyR1) mutations associated with malignant hyperthermia and their impact on skeletal excitation-contraction coupling." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25722-30, 2003 (PubMed).