M6a Antikörper

Antigen: M6a

Klonalität: Monoklonal (321)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (IHC)

Produktnummer: ABIN130545

Produktbeschreibung

Immunogen: This antibody was purified from nude mouse ascites (clone 321) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell P3X with Wister rat splenocyte immunized with mouse M6a expressing cells.

Format: Lyophilized

Isotyp: IgG2a, IgG2a kappa  (Passende Sekundärantikörper)

Klon: 321

Beschreibung: M6 is a membrane glycoprotein that is abundantly expressed on central neurons in the CHS. Two distinct murine M6 cDNA (designated M6a and M6b) whose deduced amino acid sequences are remarkably similar to that of the major CNS myelin protein PLP/DM20 are known. PLP expression is limited to the white matter. M6a appears in post-mitotic neurons of the brain and spinal cord as early as embryonic day 10, and later in the hippocampus, cerebral cortex, and the granule cells of the cerebellum. In contrast, M6b is expressed at early embryonic stage in the ventricular zone of the spinal cord, and later during development in both neurons and glia.

Spezifität: This antibody reacts with mouse M6a on flow cytometry.

Anwendungen

Applikationshinweise: Western blotting: Not tested. Immunohistochemistry: 1-10 µg/mL This antibody is suitable for use on frozen sections. Flow Cytometry: 5-10 µg/mL (final concentration)

Konzentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Flow cytometric analysis of Mouse M6a expression in LO cells. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogramindicates the reaction of ABIN130545 to the cells.

Publikationen

Publikationen: Alfonso, Fernandez, Cooper et al.: "The stress-regulated protein M6a is a key modulator for neurite outgrowth and filopodium/spine formation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 47, pp. 17196-201, 2005 (PubMed).