RFP Antikörper (Red Fluorescent Protein) (AA 234) (HRP)

Details for Product anti-RFP Antibody No. ABIN129725, Anbieter: Anmelden zum Anzeigen
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Epitop
AA 234
30
6
3
2
1
Reaktivität
Discosoma
81
50
36
9
7
2
1
1
Wirt
Kaninchen
96
36
14
9
1
Klonalität
Polyklonal
Konjugat
Dieser RFP Antikörper ist konjugiert mit HRP
9
6
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
133
69
61
53
33
29
7
4
4
3
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence derived from the mushroom polyp coral Discosoma.
Immunogentype:Recombinant
Isotyp IgG
Produktmerkmale Concentration Definition: by UV absorbance at 280 nm
Andere Bezeichnung RFP (RFP Antibody Abstract)
Hintergrund Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Synonyms: HRP, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Forschungsgebiet Tags/Labels
Applikationshinweise Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Restore with deionized water (or equivalent)
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel Gentamicin sulfate
Handhabung Do NOT add Sodium Azide!
Lagerung 4 °C
Bilder des Herstellers
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (HRP) (ABIN129725) Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. L...
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