Interleukin-1 Receptor-Associated Kinase 3 (IRAK3) Antikörper

Antigen: Interleukin-1 Receptor-Associated Kinase 3 (IRAK3)

Synonyme: ASRT5, IRAKM, IRAK-M, FLJ13601, IRAK3, IrakM, AI563835, 4833428C18Rik, si:dkey-150i15.2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129703

Produktbeschreibung

Weitere Bezeichnung: IRAK-M (Interleukin-1 Receptor-Associated Kinase M)

Gen-ID: AAD40879

Swiss-Prot: Q9Y616

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human IRAK-M protein.

Format: Liquid (sterile filtered)

Beschreibung: IRAK-M (Interleukin-1 receptor-associated kinase M). Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induce cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). Through IL-1/Toll receptors, IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF- kappa B (1,2), a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M (3). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF- kappa B activation. Members of the IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.

Spezifität: This affinity-purified antibody is directed against human IRAK-M protein. The product was purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with IRAK-M protein from human, mouse and rat sources based on 100% homology with the immunizing sequence. No cross reactivity is noted against IRAK-1 or IRAK-2. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to IRAK-M protein by western blotting in the appropriate cell lysate or extract. Note: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to IRAK-M protein by western blotting in the appropriate cell lysate or extract.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Zytokine, Rezeptoren, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Interleukin-1 Receptor-Associated Kinase 3 (IRAK3) antibody

1.0 mg/ml (by UV absorbance at 280 nm)