ECSIT Homolog (Drosophila) (ECSIT) Antikörper

Antigen: ECSIT Homolog (Drosophila) (ECSIT)

Synonyme: MGC94704, ECSIT, DmECSIT, dECSIT, DmelCG10610, CG10610, ecsit, SITPEC, MGC128961

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129700

Produktbeschreibung

Weitere Bezeichnung: ECSIT

Gen-ID: NP_057665

Swiss-Prot: Q9NYI1

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ECSIT protein.

Format: Liquid (sterile filtered)

Beschreibung: Activation of NF- kappa B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Spezifität: This affinity-purified antibody is directed against human ECSIT protein. The product was purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with ECSIT protein from human and mouse sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. Predicted Molecular Weight: Human ECSIT, MW 49.1 kDa (431 aa)

Anwendungen

Applikationshinweise: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 45 kDa in size corresponding to ECSIT protein by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:5,000 - 1:20,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Immunologie, Angeborene Immunität

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-ECSIT Homolog (Drosophila) (ECSIT) antibody