Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antikörper

Antigen: Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1)

Synonyme: S6K, PS6K, S6K1, STK14A, p70-S6K, p70-alpha, p70(S6K)-alpha

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129695

Produktbeschreibung

Weitere Bezeichnung: p70 S6 Kinase

Swiss-Prot: P23443

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p70S6K protein. Protein Sequence: Human p70S6K, 525 aa, predicted MW 59.1 kDa

Format: Liquid (sterile filtered)

Beschreibung: The protein p70 S6 kinase is critical for cell cycle progression and cell survival. In response to mitogen stimulation, p70 S6 kinase activation up-regulates ribosomal biosynthesis and enhances the translational capacity. p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome. This kinase was first characterized as an insulin/mitogen-activated protein kinase, whose major known substrate is the 40S ribosomal subunit protein S6. p70S6K is activated by diverse stimuli through multi-site phosphorylation at positions such as Thr-252 and Ser-434. In Alzheimer's Disease, p70S6K activation is associated with PHF-tau (hyperphosphorylated tau) accumulation. In non-neuronal cells, p70S6K has been shown to regulate actin polymerization.

Spezifität: This affinity-purified antibody is directed against the human p70S6K protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with p70S6K proteins from human, rat and mouse. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 70 kDa in size corresponding to p70S6K by western blotting in the appropriate cell lysate or extract.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.1 M Tris HCl, 0.15 M Sodium Chloride, pH 7.5.

Lagerung: Store vial at -20° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Signaltransduktion, Kinasen/Phosphatasen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) antibody