SMAD3 Antikörper (SMAD, Mothers Against DPP Homolog 3) (AA 417-425)

Details for Product anti-SMAD3 Antibody No. ABIN129675, Anbieter: Anmelden zum Anzeigen
Antigen
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
  • SMAD family member 3
  • MAD homolog 3a (Drosophila)
  • mothers against decapentaplegic homolog 3
  • SMAD3
  • smad3a
  • LOC100346303
  • smad3
  • Smad3
Epitop
AA 417-425, pSer425, pSer423
56
51
50
41
35
23
19
18
18
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13
12
11
11
11
9
8
8
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4
3
3
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2
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2
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2
2
2
2
1
1
1
1
1
1
1
1
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1
1
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Reaktivität
Human
533
284
265
38
29
23
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13
4
3
3
3
2
2
1
1
1
1
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Wirt
Kaninchen
468
58
23
Klonalität
Polyklonal
Konjugat
Dieser SMAD3 Antikörper ist unkonjugiert
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19
17
9
9
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8
8
5
5
5
5
5
5
3
3
1
1
1
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1
1
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1
1
1
1
Applikation
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
386
223
202
107
106
67
46
36
26
18
9
7
7
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.
Isotyp IgG
Kreuzreaktivität Xenopus laevis, Xenopus tropicalis, Zebrafisch (Brachydanio rerio), Ratte (Rattus), Maus, Schwein, Schaf, Huhn
Produktmerkmale Concentration Definition: by UV absorbance at 280 nm
Andere Bezeichnung SMAD3 (SMAD3 Antibody Abstract)
Hintergrund This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.   These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway.  Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody
Gen-ID 4088, 5174513
UniProt P84022
Forschungsgebiet Signaling, Transcription Factors
Pathways Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie
Applikationshinweise This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.  Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.5 µg/ml to detect sig...
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) 1.17 mg/ml (by UV absorbance at 280 nm)
 image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (Image 3)
 image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (Image 4)
Produkt verwendet in: Beckham, Tuttle, Tyler: "Reovirus activates transforming growth factor beta and bone morphogenetic protein signaling pathways in the central nervous system that contribute to neuronal survival following infection." in: Journal of virology, Vol. 83, Issue 10, pp. 5035-45, 2009 (PubMed).

Coffman, Coluccio, Planchart, Robertson: "Oral-aboral axis specification in the sea urchin embryo III. Role of mitochondrial redox signaling via H2O2." in: Developmental biology, Vol. 330, Issue 1, pp. 123-30, 2009 (PubMed).

Yamashita, Fatyol, Jin, Wang, Liu, Zhang: "TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta." in: Molecular cell, Vol. 31, Issue 6, pp. 918-24, 2008 (PubMed).

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