RAD52 Homolog (S. Cerevisiae) (RAD52) Antikörper

Antigen: RAD52 Homolog (S. Cerevisiae) (RAD52)

Synonyme: Rad52yh, RAD52, MGC112400, zgc:112400, LOC100223085, MGC115453

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129613

Produktbeschreibung

Weitere Bezeichnung: RAD52

Gen-ID: NP_002870

Swiss-Prot: P43351

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding to aa 360 - 375 of the Human Rad 52 protein. Protein Sequence: Human RAD52, 419 aa, predicted MW 46.3 kDa 1 msgteeailg grdshpaagg gsvlcfgqcq ytaeeyqaiq kalrqrlgpe yissrmaggg 61 qkvcyieghr vinlanemfg yngwahsitq qnvdfvdlnn gkfyvgvcaf vrvqlkdgsy 121 hedvgygvse glkskalsle karkeavtdg lkralrsfgn algncildkd ylrslnklpr 181 qlplevdltk akrqdlepsv eearynscrp nmalghpqlq qvtspsrpsh avipadqdcs 241 srslsssave seathqrklr qkqlqqqfre rmekqqvrvs tpsaekseaa ppappvthst 301 pvtvseplle kdflagvtqe liktlednse kwavtpdagd gvvkpssrad paqtsdtlal 361 nnqmvtqnrt phsvchqkpq aksgswdlqt ysadqrttgn weshrksqdm kkrkydpsy

Format: Liquid (sterile filtered)

Beschreibung: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, aprotein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bindsingle-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNAstrands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNArecombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for thisgene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, ascompared to other isoforms (beta, delta, and gamma).

Spezifität: This is an affinity purified antibody produced by immunoaffinity chromatography using theimmunizing peptide after immobilization to a solid phase. Reactivity occurs against human RAD52 protein.Cross reactivity with RAD52 protein from non-human sources is not expected based on a lack of homology (BLAST) with otherknown proteins.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in western blotting using HeLa nuclear extracts.The antibody is also functional by ELISA. Dilutions for western blotting represent a starting point dilution and furtheroptimization may be required. The antibody detects a band of approximately 63.0 kDa (predicted molecular weight: 46.3 kDa). Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with theimmunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown. ELISA 1:5,000 - 1:20,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, DNS/RNS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis is shown using antibodies-online's Affinity Purified anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~63.0 kDa is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye