V5 Epitope Tag Antikörper

Details zu Produkt Nr. ABIN129577, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
399
18
16
3
3
Wirt
Kaninchen
248
135
34
7
5
4
3
1
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
30
27
21
13
12
12
11
11
11
10
10
10
10
9
9
8
8
7
5
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
365
260
136
93
77
71
51
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.
Sequenz GKPIPNPLLG LDST
Isotyp IgG
Produktmerkmale Concentration Definition: by UV absorbance at 280 nm
Andere Bezeichnung V5-Tag
Substanzklasse Tag
Hintergrund Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG, HA and V5. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Gen-ID 55775699
Forschungsgebiet Tags/Labels
Applikationshinweise Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.73 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Bilder des Herstellers
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (ABIN129577) Anti-V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by west...
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