Phosphotyrosine (0) Antikörper

Antigen: Phosphotyrosine (0)

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129573

Produktbeschreibung

Weitere Bezeichnung: Phosphotyrosine (Free)

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.

Format: Liquid (sterile filtered)

Spezifität: This product was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG. Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphothreonine or phosphoserine.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:1,000- 1:5,000 WESTERN BLOT 1:500 IMMUNOPRECIPITATION 10.0 ug/ 500 u g sample IMMUNOHISTOCHEMISTRY 1:50 OTHER APPLICATIONS User Optimized. Note: This affinity-purified antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays. The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Konzentration: 1.0 mg/ml

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.

Lagerung: Store vial at -20° C prior to opening. Aliquot contents and store at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Aminosäuren, Proteinmodifikationen

Beschränkungen: Nur für Forschungszwecke einsetzbar