Programmed Cell Death Protein 8 (AIFM1) Antikörper

Antigen: Programmed Cell Death Protein 8 (AIFM1)

Synonyme: AIF, PDCD8, MGC111425

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Human, Ratte (Rattus)

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129543

Produktbeschreibung

Weitere Bezeichnung: Apoptosis Inducing Factor (AIF)

Swiss-Prot: O95831

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at an internal region of human AIF protein.

Format: Liquid (sterile filtered)

Beschreibung: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like cytochrome c and caspase-9, which are critical molecules in apoptosis, AIF localizes to mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis. These effects occur in both isolated nuclei and in the nuclei of live cells treated by microinjection and with apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and is widely expressed (2).

Spezifität: This antibody is directed against human AIF protein. The product was purified from monospecific antiserum by DEAE ion exchange chromatography. A BLAST analysis was used to suggest cross-reactivity with AIF protein from mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: This antibody has been tested for use in western blotting and immunohistochemistry. K562 cell lysate, as well as rat and mouse heart tissue lysates, can be used as positive controls in western blotting, and a band at approximately 67 kDa is expected. Specific conditions for reactivity should be optimized by the end user.

Konzentration: 0.5 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Apoptose/Nekrose

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody at 1 ug/ml.

Immunohistochemistry of AIF in human retina with AIF antibody at 10 ug/ml.