beta-Site APP-Cleaving Enzyme 1 (BACE) Antikörper

Antigen: beta-Site APP-Cleaving Enzyme 1 (BACE)

Synonyme: ASP2, BACE, HSPC104, FLJ90568, KIAA1149, C76936, Bace, bace1, MGC77409, BACE1, MGC137940, MGC145931, Bace1, ASP1, BAE2, DRAP, AEPLC, ALP56, ASP21, CDA13, CEAP1, ARP1, AI850424, 1110059C24Rik, bace2, MGC68881, MGC68482, BACE2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Human, Ratte (Rattus)

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129542

Produktbeschreibung

Weitere Bezeichnung: BACE/Asp2 (Beta-Site APP Cleaving Enzyme)

Gen-ID: AAF04142

Swiss-Prot: P56817

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human BACE protein.

Format: Liquid (sterile filtered)

Beschreibung: Accumulation of the amyloid- beta (A beta ) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. A beta peptide is generated by proteolytic cleavage of the beta -amyloid protein precursor (APP) at beta - and gamma -sites by two proteases. APP is first cleaved by beta -secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as the substrate for gamma -secretase to generate the 4 kDa amyloid- beta peptide, which is deposited in the brains of all sufferers of Alzheimer's disease. The long-sought beta -secretase was recently identified by several groups independently and designated beta -site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) (1-4). BACE/Asp2 is a novel transmembrane aspartic protease and co-localizes with APP.

Spezifität: This antibody is directed against human BACE protein. The product was purified from monospecific antiserum by DEAE ion exchange chromatography. The antibody is human and mouse reactive. Reactivity against homologues from other sources has not been determined.

Synonyme: ASP2, BACE, HSPC104, FLJ90568, KIAA1149, C76936, Bace, bace1, MGC77409, BACE1, MGC137940, MGC145931, Bace1, ASP1, BAE2, DRAP, AEPLC, ALP56, ASP21, CDA13, CEAP1, ARP1, AI850424, 1110059C24Rik, bace2, MGC68881, MGC68482, BACE2

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA User Optimized WESTERN BLOT 0.5-1 ug/ml IMMUNOCYTOCHEMISTRY 10 ug/ml OTHER APPLICATIONS User Optimized. Note: This antibody has been tested for use in western blotting and immunohistochemistry. Human brain tissue can be used as a positive control in western blotting, and a band at approximately 70 kDa is expected. Specific conditions for reactivity should be optimized by the end user.

Konzentration: 0.5 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of BACE in human brain tissue lysate in the absence (A) or presence (B) of blocking peptide, and in mouse 3T3 cell lysate (C) with BACE antibody at 1 ug/ml.

Immunocytochemistry of BACE in 3T3 cells with BACE antibody at 10 ug/ml.