CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antikörper

Antigen: CASP8 and FADD-Like Apoptosis Regulator (CFLAR)

Synonyme: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, Cash, Flip, Gm9845, c-Flip, AI646576, AU021929, MGC103395, 2310024N18Rik, A430105C05Rik, ENSMUSG00000072980, C-FLIP, MGC108616, Clarp1, CFLAR, DKFZp469E1815

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Human, Ratte (Rattus)

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129541

Produktbeschreibung

Weitere Bezeichnung: FLICE Inhibitory Protein alpha (FLIPalpha)

Gen-ID: NP_003870

Swiss-Prot: O15519

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of the human FLIP alpha /FLIP L protein .

Format: Liquid (sterile filtered)

Beschreibung: Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively (1,2). The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP (3-7). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of FLIPs have been identified and termed FLIP alpha , beta , gamma , and delta , respectively (8).

Spezifität: This antibody is directed against human FLIP alpha protein. The product was purified from monospecific antiserum by DEAE ion exchange chromatography. The antibody is human, mouse and rat reactive and is specific for the alpha splice variant. Reactivity against homologues from other sources has not been determined.

Synonyme: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, Cash, Flip, Gm9845, c-Flip, AI646576, AU021929, MGC103395, 2310024N18Rik, A430105C05Rik, ENSMUSG00000072980, C-FLIP, MGC108616, Clarp1, CFLAR, DKFZp469E1815

Anwendungen

Applikationshinweise: This antibody has been tested for use in western blotting and immunohistochemistry. HeLa whole cell lysate can be used as a positive control in western blotting, and a band at approximately 55 kDa is expected. Specific conditions for reactivity should be optimized by the end user.

Konzentration: 0.5 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysates with FLIP antibody at 0.5 ug/ml.

Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 10 ug/ml.