SLCO1B1 / OATP2 (+ OATP8) Antikörper

Antigen: SLCO1B1 / OATP2 (+ OATP8)

Klonalität: Monoklonal (MMDQ)

Wirt: Maus

Reaktivität: Human

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN126013

Produktbeschreibung

Produktmerkmale: Synonyms: LST1, LST-1, OATP-2, OATPC, OATP-C, SLC21A6, Solute carrier family 21 member 6,Liver-specific organic anion transporter 1, Solute carrier organic anion transporter familymember 1B1, Sodium-independent organic anion-transporting polypeptide 2

Gen-ID: 10599

Swiss-Prot: Q9Y6L6

Immunogen: Synthetic N-terminus (24 aa) of Human organic anion transporter OATP2 coupled to KLH

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MMDQ

Spezifität: In Western blot analyses of basolateral membrane fractions of human liver the monoclonalantibody mMDQ reacts with 90 kD and 120 kD proteins (after SDS-PAGE) representingOATP2 (also described as OATP-C) and OATP8, respectively. According to a recent newnomenclature these hepatocyte-specific proteins are identical to OATP1B1 and OATP1B3,respectively (see Ref. 2). Using immunofluorescence microscopy mMDQ stains the sinusoidal (basolateral)membranes in frozen sections of human liver, hepatocellular carcinoma (see Ref. 1). Reacts with OATP2 and OATP8, human organic anion transporters (90 kD, 120 kD, afterSDS-PAGE). Reacts with Transfected HEK293 cells, negative with HepG2 and PLC. Species Reactivity: Tested: Human.

Anwendungen

Protokoll: Western Blotting: (Ref. 1)1. For optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is notrecommended, alternatively, samples can be kept at 37°C for 30 min2. 50 µg of protein lysate should be loaded on each lane. 3. The usage of a PVDF-Membrane is recommended. 4. The usage of 10% milk powder in TTBS (Tris-buffered saline, pH 7. 6, with 0. 05% Tween20) as blocking reagent. The antibody can be diluted 3-5 x

Applikationshinweise: Immunoprecipitation. Immunofluorescence Microscopy. Western blotting: For optimal detection of the glycosylated antigen sample boiling beforeSDS-PAGE is not recommended. Alternatively, samples can be kept at 37°C for 30 min. (SeeRef.1)Immunohistochemistry on Paraffin Sections (only after microwave treatment: useundiluted. For details See Ref.1). Incubation Time: 1 h at RT, for Paraffin Sections overnight at 2-8°C. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Lagerung: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Hagenbuch, Meier: "The superfamily of organic anion transporting polypeptides." in: Biochimica et biophysica acta, Vol. 1609, Issue 1, pp. 1-18, 2002 (PubMed).