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CD16 Antikörper

CD16 Reaktivität: Human FACS, IP, WB, Func Wirt: Maus Monoclonal MEM-154 unconjugated
Produktnummer ABIN125685
  • Target Alle CD16 Antikörper anzeigen
    CD16
    Reaktivität
    • 144
    • 26
    • 21
    • 21
    • 21
    • 9
    • 8
    • 8
    • 8
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 128
    • 29
    • 14
    • 1
    Maus
    Klonalität
    • 144
    • 26
    • 1
    Monoklonal
    Konjugat
    • 59
    • 19
    • 13
    • 13
    • 9
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD16 Antikörper ist unkonjugiert
    Applikation
    • 137
    • 31
    • 31
    • 26
    • 24
    • 23
    • 22
    • 20
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)
    Spezifität
    The antibody MEM-154 reacts with an extracellular epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes, and it can be used for mapping CD16-158V/F polymorphism on NK cells, as it requires presence of V at amino acid 158.
    Kreuzreaktivität (Details)
    Human
    Aufreinigung
    Purified by protein-A affinity chromatography.
    Reinheit
    > 95 % (by SDS-PAGE)
    Immunogen
    Human granulocytes
    Klon
    MEM-154
    Isotyp
    IgG1
    Top Product
    Discover our top product CD16 Primärantikörper
  • Applikationshinweise
    Functional application: The antibody MEM-154 blocks binding of human IgG to FcγRIII.
    Flow cytometry: Recommended dilution: 5-10 μg/mL, positive control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.
    Western blotting: Non-reducing conditions.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4
    Konservierungsmittel
    Azide free
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze.
  • Gasdaska, Sherwood, Regan, Dickey: "An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab." in: Molecular immunology, Vol. 50, Issue 3, pp. 134-41, (2012) (PubMed).

    de Haas, Koene, Kleijer, de Vries, Simsek, van Tol, Roos, von dem Borne: "A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2948-55, (1996) (PubMed).

    Tamm, Schmidt: "The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 4, pp. 1576-81, (1996) (PubMed).

  • Target
    CD16
    Andere Bezeichnung
    CD16 (CD16 Produkte)
    Synonyme
    Fcgr3 antikoerper, Fcgr3a antikoerper, CD16 antikoerper, FCGR3 antikoerper, FCGR3@ antikoerper, CD16b antikoerper, FCG3 antikoerper, FCR-10 antikoerper, FCRIII antikoerper, FCRIIIb antikoerper, FcRIII antikoerper, Fc fragment of IgG, low affinity IIa, receptor antikoerper, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antikoerper, Fc fragment of IgG receptor IIIb antikoerper, Fc fragment of IgG receptor IIIa antikoerper, Fc receptor, IgG, low affinity III antikoerper, Fc-gamma RIII antikoerper, Fcgr2a antikoerper, FCGR3A antikoerper, FCGR3B antikoerper, Fcgr3 antikoerper, FCGR3 antikoerper
    Hintergrund
    CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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