DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antikörper

Antigen: DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA)

Synonyme: DFF1, ICAD, DFF-45, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, DFFA, MGC143066, DKFZp469D0625

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125653

Produktbeschreibung

Weitere Bezeichnung: DFF-45

Immunogen: A synthetic peptide derived from N-terminus of human DFF-45 protein.

Format: Ig-fraction

Spezifität: Apoptosis is induced through a number of cell death receptors and their ligands like TNF and TNF receptor. The signaling cascade is carried further by adaptor proteins containing death domains and by caspase family of proteins. DFF45 and DFF40 (ICAD and CAD) are two subunits of heterodimeric caspase activated DNAse. DFF45/ ICAD (Inhibitor of Caspase Activated DNAse) acts as a chaperon for appropriate folding of DFF40 and it also acts as inhibitor of DFF40. Caspase 3 upon activation cleaves DFF to release DFF40/CAD, which oligomerizes into a large active complex and eventually causes the degradation of DNA in nuclei. Hence cleavage of DFF45 is an important step in apoptosis.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against DFF-45 containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Apoptose/Nekrose, DNS/RNS

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human Colon carcinoma stained with DFF-45 antibody