alpha-Methylacyl-CoA Racemase (AMACR) Antikörper

Antigen: alpha-Methylacyl-CoA Racemase (AMACR)

Synonyme: RACE, CBAS4, Macr1, Da1-8, Marc1, AMACR, DKFZp469O1232, amacr, MGC89832

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125649

Produktbeschreibung

Weitere Bezeichnung: P504S alpha-Methylacyl-CoA Racemace (AMACR)

Immunogen: A synthetic human AMACR peptide.

Format: Ig-fraction

Spezifität: P504S has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of P504S protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. P504S can be used as a positive marker for PIN.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Prostate carcinoma. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against AMACR containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human tonsil stained with P504S antibody

Publikationen

Publikationen: Morikawa, Goto, Tomita et al.: "Recurrent prostatic stromal sarcoma with massive high-grade prostatic intraepithelial neoplasia." in: Journal of clinical pathology, Vol. 60, Issue 3, pp. 330-2, 2007 (PubMed).