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Tumor Protein P53 (TP53) Antikörper

Antigen

Tumor Protein P53 (TP53)

Synonyme p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Klonalität Polyklonal
Wirt
Alternativen

Kaninchen

Reaktivität
Alternativen

Human, Maus, Ratte (Rattus)

Applikation
Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p))
Produktnummer ABIN125624
Menge 500µl  (100ug/ml approximately)  (Varianten)
Preis 151,92 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung P53
Immunogen Recombinant full-length wild type p53 protein.
Format Ig-fraction, Purified
Spezifität This antibody reacts with a 53 kD protein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types. It is involved in regulating cell growth, replication and apoptosis. It binds to mdm2, SV40 T antigen and human Papilloma virus E6 protein. Mutation involving p53 is found in wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung and melanoma. This antibody crossreacts with mouse and rat.

Anwendungen

Applikationshinweise Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Nuclear
Konzentration 100ug/ml approximately
Buffer Purified immunoglobulin fraction of rabbit antiserum against p53 containing sodium azide as preservative.
Lagerung Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Forschungsgebiet Transkriptionsfaktoren
Beschränkungen Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Alternativen

Alternativen zu Antigen "Tumor Protein P53 (TP53)", Typ "Antikörper" finden
Wirte Kaninchen (310), Maus (156), Schaf (4), Huhn (1), Ratte (1)
Reaktivitäten Human (439), Maus (94), Ratte (Rattus) (85), Rind (Kuh) (14), Katze (10), Pferd (10), Affe (8), Kaninchen (6), Hund (5), Schwein (4), Primaten (4), Schaf (4), Hamster (3), Primate (3), Xenopus laevis (2), Vogel (1)
Applikationen Western Blot (WB) (383), Immunhistochemie (Paraffinschnitte) (IHC (p)) (142), Immunpräzipitation (IP) (94), Immunhistochemie (IHC) (90), Immunfluoreszenz (IF) (80), ELISA (76), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (75), Enzyme Immunoassay (EIA) (55), Durchflusszytometrie (FACS) (43), Immunzytochemie (ICC) (15), Dot Blot (Dot) (10), Radioimmunoassay (RIA) (4), Transfizierte Zellkultur (TCC) (3), Chromatin-Immunopräzipitation (ChiP) (1)
Konjugate FITC (11), Biotin (4), PE (3), HRP (1)
Epitope pSer392 (18), pSer315 (12), pSer37 (11), pSer46 (11), pSer33 (10), pSer15 (9), pSer6 (9), pThr18 (9), AA 20-25 (5), AA 371-380 (5), C-Term (5), pSer20 (5), pSer32/Ser36 (5), pSer1232 (4), AA 18-30 (3), AA 46-55 (3), pSer9 (3), Lys305 (2), Lys382 (2), S315 (2), S37 (2), S46 (2), T55 (2), phospho (2), AA 16-30 (1), AA 378-393 (1), AA 46-53 (1), Acetyl-Lys317 (1), Acetyl-Lys379 (1), N-Term (1), S15 (1), S376 (1), S378 (1), S9 (1), T18 (1), amino acids 211-220 (1), pS20 (1), pS33 (1), pSer376 (1), pSer378 (1), pThr55 (1)