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Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) Antikörper

Antigen

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2)
Synonyme GAD65, MGC161605, MGC161607, Gad-2, GAD(65), 6330404F12Rik, gad65, zgc:112198, GAD2, F12P19.12, F12P19_12, GLUTAMATE DECARBOXYLASE 2
Klonalität Polyklonal
Wirt
Alternativen

Kaninchen
Reaktivität
Alternativen

Human, Ratte (Rattus)
Applikation
Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p))
Produktnummer ABIN125594
Menge 500µl  (100ug/ml approximately)  (Varianten)
Preis 300,96 €   Zzgl. Versandkosten €20,00 und MWSt
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Produktbeschreibung
Anwendungen Alternativen Sekundärantikörper Varianten zurück nach oben

Weitere Bezeichnung Glutamate Decarboxylase 65 (GAD65)
Immunogen A synthetic peptide derived from the C-terminus region of the human GAD65 protein.
Format Ig-fraction, Purified
Spezifität This antibody reacts with a 65 kD protein, known as GAD (Glutamate Decarboxylase). Two forms of GAD exist in adults, GAD65 and GAD67, which are encoded by different genes. GAD65 and GAD67 are expressed in most GABA containing neurons. GAD65 appears to be targeted to the membrane and nerve ending, where as GAD67 is more widely distributed throughout the cell. This antibody cross reacts with rat.

Anwendungen
Alternativen Sekundärantikörper Varianten zurück nach oben

Applikationshinweise Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreas. Cellular localization: Cytoplasmic
Konzentration 100ug/ml approximately
Buffer Purified immunoglobulin fraction of rabbit antiserum against human GAD65 containing sodium azide as preservative.
Lagerung Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Forschungsgebiet Enzyme, Metabolismus
Beschränkungen Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Alternativen
Anwendungen Sekundärantikörper Varianten zurück nach oben

Alternativen zu Antigen "Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2)", Typ "Antikörper" finden
Wirte Kaninchen (59), Ziege (6), Maus (2)
Reaktivitäten Human (63), Ratte (Rattus) (49), Maus (42), Schwein (32), Huhn (30), Rind (Kuh) (20), Hund (20), Pferd (2), Schimpanse (1), Affe (1), Kaninchen (1), Schaf (1)
Applikationen Immunfluoreszenz (IF) (40), Durchflusszytometrie (FACS) (36), Western Blot (WB) (24), Immunhistochemie (Paraffinschnitte) (IHC (p)) (11), ELISA (10), Immunpräzipitation (IP) (6), Enzyme Immunoassay (EIA) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (2), Radioimmunoassay (RIA) (2), Dot Blot (Dot) (1), Immunzytochemie (ICC) (1), Immunhistochemie (IHC) (1)
Konjugate Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)