CD95 Antikörper
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (153), Maus (17), Ratte (Rattus) (9), Affe (7), Katze (3), Hund (3), Rind (Kuh) (1), Fruchtfliege (Drosophila melanogaster) (1), Hamster (1), Schwein (1), Kaninchen (1), Schaf (1), Xenopus laevis (1)
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Applikation
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Durchflusszytometrie (FACS) (119), Western Blot (WB) (39), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (37), Immunpräzipitation (IP) (36), Immunhistochemie (Paraffinschnitte) (IHC (p)) (24), Enzyme Immunoassay (EIA) (16), Funktionale Studien (Func) (15), Immunfluoreszenz (IF) (9), Blockierende Antikörper (Inhibition) (8), ELISA (7), Apoptose-Induktion (AI) (4), Immunhistochemie (IHC) (3), Dot Blot (Dot) (1), Neutralisierung (Neut) (1)
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| Produktnummer |
ABIN125585 |
| Menge |
500µl (100ug/ml approximately) (Varianten) |
| Preis |
300,96 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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CD95 (Fas)
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Immunogen
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A synthetic peptide from the C-terminus of human Fas protein.
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Format
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Ig-fraction, Purified
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Spezifität
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This antibody reacts with a 48 kD transmembrane glycoprotein, known as CD95 (Fas). The Fas antigen is expressed on the surface of various cell types, including activated T and B lymphocytes and T lymphoblastoid cell lines. CD95 mediates receptor-triggered apoptosis.
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Applikationshinweise
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Small intestine. Cellular localization: Cell membrane
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Konzentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against human CD95 containing sodium azide as preservative.
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Lagerung
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Forschungsgebiet
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CD Antigene
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Beschränkungen
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Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
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Alternativen zu Antigen "CD95", Typ "Antikörper" finden
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Wirte
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Maus (131), Kaninchen (25), Ratte (6), Huhn (2), Ziege (1)
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Reaktivitäten
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Human (153), Maus (17), Ratte (Rattus) (9), Affe (7), Katze (3), Hund (3), Rind (Kuh) (1), Fruchtfliege (Drosophila melanogaster) (1), Hamster (1), Schwein (1), Kaninchen (1), Schaf (1), Xenopus laevis (1)
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Applikationen
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Durchflusszytometrie (FACS) (119), Western Blot (WB) (39), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (37), Immunpräzipitation (IP) (36), Immunhistochemie (Paraffinschnitte) (IHC (p)) (24), Enzyme Immunoassay (EIA) (16), Funktionale Studien (Func) (15), Immunfluoreszenz (IF) (9), Blockierende Antikörper (Inhibition) (8), ELISA (7), Apoptose-Induktion (AI) (4), Immunhistochemie (IHC) (3), Dot Blot (Dot) (1), Neutralisierung (Neut) (1)
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Konjugate
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FITC (21), PE (18), APC (8), Biotin (8), Alexa Fluor 488 (2), SPRD (2), Alexa Fluor 647 (1), PE,Cy5 (1)
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Epitope
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C-Term (4), Center (1), pTyr232 (1), pTyr291 (1)
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