Antigen Identified By Monoclonal Antikörper Ki-67 (MKI67) Antikörper

Antigen: Antigen Identified By Monoclonal Antibody Ki-67 (MKI67)

Synonyme: KIA, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125565

Produktbeschreibung

Weitere Bezeichnung: Ki-67 Antigen

Immunogen: Synthetic peptide from 62 base pair region of the human Ki-67 antigen.

Format: Ig-fraction

Spezifität: This antibody reacts with a nuclear antigen present in proliferating human cells. This antibody cross-reacts with a range of mammalian species. It can be used to evaluate the ki- 67 labeling index in various tumors.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Konzentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human Ki-67 antigen containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Proliferationsmarker, Zellzyklus

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody

Publikationen

Publikationen: Song, Kojima, Hanamura et al.: "Expression of drebrin E in migrating neuroblasts in adult rat brain: coincidence between drebrin E disappearance from cell body and cessation of migration." in: Neuroscience, Vol. 152, Issue 3, pp. 670-82, 2008 (PubMed).

Franco, Chiesa, Beloti et al.: "Human osteoblastic cell response to a Ca- and P-enriched titanium surface obtained by anodization." in: Journal of biomedical materials research. Part A, Vol. 88, Issue 4, pp. 841-8, 2009 (PubMed).

de Oliveira, de Oliva, Maximiano et al.: "Effects of a mixture of growth factors and proteins on the development of the osteogenic phenotype in human alveolar bone cell cultures." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 56, Issue 7, pp. 629-38, 2008 (PubMed).