CD3 (CD3E) Antikörper

Antigen: CD3 (CD3E)

Synonyme: T3E, TCRE, FLJ18683

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125550

Produktbeschreibung

Weitere Bezeichnung: CD3

Immunogen: Synthetic human CD3 polypeptide conjugated to bovine serum albumin.

Format: Ig-fraction

Spezifität: This antibody reacts with intracytoplasmic portion of the CD3 antigen on the T cells. It stains T cells in cortex as well as medulla of the thymus and lymphoid tissues. This antibody also labels T cell neoplasm, malignant histiocytes and in Hodgkin's disease.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Konzentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human T cell containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: CD Antigene

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human tonsil stained with CD3 antibody