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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antikörper

Antigen

zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyme SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70
Klonalität Monoklonal (2F3-2)
Wirt
Reaktivität
Alternativen

Human

Applikation
Alternativen Immunhistochemie (IHC)
Produktnummer ABIN125522
Menge 500ul  (100ug/ml approximately)  (Varianten)
Preis 246,15 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung ZAP-70
Immunogen GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254.
Isotyp IgG2a  (Passende Sekundärantikörper)
Klon 2F3-2
Spezifität Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course. Recent studies suggest that protein levels of ZAP-70 are elevated in B-cells of CLL patients with non-mutant heavy-chain variable region (IgVH) but not those with the mutant regions. Recent evidence suggests that ZAP-70 could be an excellent prognostic biomarker with high levels of the proteins indicating a poor prognosis.

Anwendungen

Applikationshinweise Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasmic
Konzentration 100ug/ml approximately
Buffer This antibody is supplied as liquid containing sodium azide as preservative.
Lagerung Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Forschungsgebiet Signaltransduktion, Tags/Labels, Immunologie, Krebs
Beschränkungen Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Alternativen

Alternativen zu Antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", Typ "Antikörper" finden
Wirte Kaninchen (116), Maus (73)
Reaktivitäten Human (186), Maus (94), Ratte (Rattus) (68), Rind (Kuh) (18), Schwein (18), Hund (17), Pferd (16), Kaninchen (16), Huhn (15), Meerschweinchen (15)
Applikationen Western Blot (WB) (101), Durchflusszytometrie (FACS) (58), Immunhistochemie (Paraffinschnitte) (IHC (p)) (35), Immunfluoreszenz (IF) (34), ELISA (32), Immunhistochemie (IHC) (32), Immunpräzipitation (IP) (23), Enzyme Immunoassay (EIA) (21), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Intrazelluläre Durchflusszytometrie (ICFC) (6), Immunzytochemie (ICC) (4), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
Konjugate FITC (11), PE (10), Biotin (9), Alkaline Phosphatase (AP) (4), HRP (3), PerCP,Cy5.5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
Epitope pTyr319 (10), pTyr493 (8), Ab-319 (5), Ab-493 (5), C-Term (5), pTyr292 (4), Center (3), AA 319-602 (1), Ab-292 (1), Ab-315 (1), pTyr315 (1), pTyr492 (1), pYTyr315/319 (1)