»
Antikörper
»
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antikörper
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antikörper
| Antigen |
|
| Synonyme |
SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70 |
| Klonalität |
Monoklonal (2F3-2) |
|
Wirt
|
|
|
Reaktivität
|
|
|
Applikation
|
Western Blot (WB) (101), Durchflusszytometrie (FACS) (58), Immunhistochemie (Paraffinschnitte) (IHC (p)) (35), Immunfluoreszenz (IF) (34), ELISA (32), Immunhistochemie (IHC) (32), Immunpräzipitation (IP) (23), Enzyme Immunoassay (EIA) (21), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Intrazelluläre Durchflusszytometrie (ICFC) (6), Immunzytochemie (ICC) (4), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
|
| Produktnummer |
ABIN125522 |
| Menge |
500ul (100ug/ml approximately) (Varianten) |
| Preis |
246,15 € Zzgl. Versandkosten €20,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 5 Werktagen |
|
Weitere Bezeichnung
|
ZAP-70
|
|
Immunogen
|
GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254.
|
|
Isotyp
|
IgG2a (Passende Sekundärantikörper)
|
|
Klon
|
2F3-2
|
|
Spezifität
|
Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course. Recent studies suggest that protein levels of ZAP-70 are elevated in B-cells of CLL patients with non-mutant heavy-chain variable region (IgVH) but not those with the mutant regions. Recent evidence suggests that ZAP-70 could be an excellent prognostic biomarker with high levels of the proteins indicating a poor prognosis.
|
|
Applikationshinweise
|
Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasmic
|
|
Konzentration
|
100ug/ml approximately
|
|
Buffer
|
This antibody is supplied as liquid containing sodium azide as preservative.
|
|
Lagerung
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Forschungsgebiet
|
Signaltransduktion, Tags/Labels, Immunologie, Krebs
|
|
Beschränkungen
|
Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
|
Alternativen zu Antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", Typ "Antikörper" finden
|
Wirte
|
Kaninchen (116), Maus (73)
|
|
Reaktivitäten
|
Human (186), Maus (94), Ratte (Rattus) (68), Rind (Kuh) (18), Schwein (18), Hund (17), Pferd (16), Kaninchen (16), Huhn (15), Meerschweinchen (15)
|
|
Applikationen
|
Western Blot (WB) (101), Durchflusszytometrie (FACS) (58), Immunhistochemie (Paraffinschnitte) (IHC (p)) (35), Immunfluoreszenz (IF) (34), ELISA (32), Immunhistochemie (IHC) (32), Immunpräzipitation (IP) (23), Enzyme Immunoassay (EIA) (21), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Intrazelluläre Durchflusszytometrie (ICFC) (6), Immunzytochemie (ICC) (4), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
|
|
Konjugate
|
FITC (11), PE (10), Biotin (9), Alkaline Phosphatase (AP) (4), HRP (3), PerCP,Cy5.5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
|
|
Epitope
|
pTyr319 (10), pTyr493 (8), Ab-319 (5), Ab-493 (5), C-Term (5), pTyr292 (4), Center (3), AA 319-602 (1), Ab-292 (1), Ab-315 (1), pTyr315 (1), pTyr492 (1), pYTyr315/319 (1)
|