alpha-Methylacyl-CoA Racemase (AMACR) Antikörper

Antigen: alpha-Methylacyl-CoA Racemase (AMACR)

Synonyme: RACE, CBAS4, Macr1, Da1-8, Marc1, AMACR, DKFZp469O1232, amacr, MGC89832

Klonalität: Monoklonal (4A4)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125518

Produktbeschreibung

Weitere Bezeichnung: PIN Cocktail P504S+p63

Immunogen: A recombinant protein derived from amino acid 1-205 of human p63. A synthetic human AMACR peptide.

Format: Supernatant + Rb / Ig Fraction

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: 4A4

Spezifität: P504S has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of P504S protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. P504S can be used as a positive marker for PIN. The p63 gene is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkable divergent abilities to transactivate p53 reporter genes and induce apoptosis. The combination of P504S and p63 (PIN) may be extremely useful for diagnosing prostatic intraepithelial neoplasia (PIN). P504S stains cytoplasm in prostatic adenocarcinomas and atypical hyperplasia and p63 stains nuclei in normal and benign prostate glands.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Prostate carcinoma. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as a cocktail of tissue culture supernatant (p63) and purified immunoglobulin fraction of rabbit antiserum (P504S) containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human prostate carcinoma stained with PIN Cocktail antibody