CD3 (CD3E) Antikörper

Antigen: CD3 (CD3E)

Synonyme: T3E, TCRE, FLJ18683

Klonalität: Monoklonal (F7-2-38)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125514

Produktbeschreibung

Weitere Bezeichnung: CD3

Immunogen: Purified CD3 epsilon gamma delta/CD3 omega

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: F7-2-38

Spezifität: CD3 is first detectable in early Thymocytes and its appearance probably represents one of the earliest signs of commitment to T cell lineage. In cortical Thymocytes, the antigen is predominantly present as an intracytoplasmic constituent. This antibody labels CD3 epsilon and is useful tool for the identification of T cells and related neoplasms.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 1 mM EDTA buffer, pH 9.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Membrane and cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: CD Antigene

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.