Paxillin (PXN) Antikörper

Antigen: Paxillin (PXN)

Synonyme: FLJ16691, Pax, AW108311, AW123232, CG18061, CG18576, CT40481, CT42454, DPaxillin, DPxn, DPxn37, Dpax, PDLP, DmelCG31794, CG31794, LOC397826, wu:fw71f12, PXN, DKFZp469J2119, LOC100220858

Klonalität: Monoklonal (5H11)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125485

Produktbeschreibung

Weitere Bezeichnung: Paxillin

Immunogen: BALB/C mice were immunized with purified full-length recombinant human paxillin protein.

Format: Purified

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: 5H11

Spezifität: This antibody with a protein of 68 kD. Paxillin is a focal adhesion protein and a substrate for several tyrosine kinase such as src, FAK and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation. This antibody cross reacts with rat.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Cell membrane, cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human colon carcinoma stained with Paxillin antibody