Transglutaminase II Antikörper

Antigen: Transglutaminase II

Klonalität: Monoklonal (CUB 7402)

Wirt: Maus

Reaktivität: Human, Hund, Kaninchen, Meerschweinchen

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125457

Produktbeschreibung

Immunogen: BALB/C mice were injected with purified guinea pig liver TGase II.

Format: Ig-fraction

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: CUB 7402

Spezifität: This antibody reacts with a 77-85 kD protein known as Transglutaminase II (TGase II). TGase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. Different tissue and cell types express varying amounts of tissue Transglutaminase. This antibody cross-reacts with dog, rabbit and Guinea pig.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic, cell membrane, extracellular matrix

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Enzyme, Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human breast carcinoma stained with Transglutaminase II antibody