Minichromosome Maintenance Complex Component 7 (MCM7) Antikörper

Antigen: Minichromosome Maintenance Complex Component 7 (MCM7)

Synonyme: MCM2, CDC47, HsMcm7, P85MCM, P1CDC47, PNAS-146, CDABP0042, P1.1-MCM3, MGC93853, MCM7

Klonalität: Monoklonal (47DC141)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Hund, Xenopus laevis

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN125445

Produktbeschreibung

Weitere Bezeichnung: cdc47 (MCM7)

Immunogen: BALB/C mice were injected with recombinant human CDC47 protein.

Format: Ig-fraction

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 47DC141

Spezifität: This antibody reacts with a 80 kD protein known as hCDC47. hCDC47 is a member of MCM family, which allows DNA to replicate once per cell cycle. Expression of hCDC47 protein has been found in the nuclei of proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contain more nuclear hCDC47-positive cells than their normal counterparts. This antibody cross reacts with mouse, rat, dog and Xenopus.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Western Blotting

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Zellzyklus

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human tonsil stained with CDC47 antibody