X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) Antikörper

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1)

Synonyme: RCC, Xrcc-1, MGC102556, xrcc1, MGC80909, XRCC1

Klonalität: Monoklonal (02-05-1933)

Wirt: Maus

Reaktivität: Human, Ratte (Rattus)

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125431

Produktbeschreibung

Weitere Bezeichnung: X-Ray Repair Cross Complementing (XRCC1)

Immunogen: BALB/C mice were injected with recombinant human XRCC1 protein.

Format: Ig-fraction

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: 02-05-1933

Spezifität: This antibody is specific to 85kD kD protein known as XRCC1. X-ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNS ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half. In testis XRCC1 is expressed at high levels. This antibody cross reacts with rat.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Testis. Cellular localization: Nuclear Other Applications: Western Blotting, Immunoprecipitation, Immunofluorescence

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: DNS/RNS

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human testis stained with XRCC-1 antibody