Prohibitin (PHB) Antikörper
| Antigen |
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| Synonyme |
PHB1, Bap32, sr:nyz128, wu:fa18f07, wu:fb09f05, phb1, MGC53103, MGC75944, PHB, MGC127238, Phb, ACYPI000080, phb |
| Klonalität |
Monoklonal (II-14-10) |
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Wirt
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Reaktivität
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Applikation
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Western Blot (WB) (39), Immunfluoreszenz (IF) (24), Durchflusszytometrie (FACS) (22), Immunhistochemie (Paraffinschnitte) (IHC (p)) (16), ELISA (13), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (6), Immunhistochemie (IHC) (6), Immunpräzipitation (IP) (6), Enzyme Immunoassay (EIA) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN125429 |
| Menge |
500ul (100ug/ml approximately) (Varianten) |
| Preis |
300,96 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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Prohibitin
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Immunogen
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BALB/C mice were injected with purified recombinant rat prohibitin protein.
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Format
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Ig-fraction
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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II-14-10
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Spezifität
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This antibody is specific to 30 kD protein known as Prohibitin. Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression. This antibody cross reacts with mouse, rat and pig.
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Applikationshinweise
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Heart. Cellular localization: Cytoplasmic Other Applications: Western Blotting, Immunoprecipitation, Immunofluorescence
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Konzentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Lagerung
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Beschränkungen
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Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
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Alternativen zu Antigen "Prohibitin (PHB)", Typ "Antikörper" finden
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Wirte
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Kaninchen (52), Maus (10)
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Reaktivitäten
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Human (55), Maus (34), Ratte (Rattus) (32), Hund (16), Pferd (15), Huhn (2), Schwein (2), Rind (Kuh) (1)
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Applikationen
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Western Blot (WB) (39), Immunfluoreszenz (IF) (24), Durchflusszytometrie (FACS) (22), Immunhistochemie (Paraffinschnitte) (IHC (p)) (16), ELISA (13), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (6), Immunhistochemie (IHC) (6), Immunpräzipitation (IP) (6), Enzyme Immunoassay (EIA) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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C-Term (4), Center (2), N-Term (2), C-Term E194 (1)
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Alternativen 
