Thrombospondin (TSP) Antikörper

Antigen: Thrombospondin (TSP)

Synonyme: CT31613, D-TSP, DTSP, SP295, tsp, DmelCG11326, CG11326, GB13833

Klonalität: Monoklonal (A6-1)

Wirt: Maus

Reaktivität: Human, Rind (Kuh), Schwein, Pferd, Hund, Schaf, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125422

Produktbeschreibung

Immunogen: BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

Format: Ig-fraction

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: A6-1

Spezifität: This antibody is specific to 450 kD protein, a non-reduced and 170 to 180 kD, reduced form of Thrombospondin. Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. This antibody cross reacts with human, cow, pig, horse, dog, sheep, mouse and rat.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Secretory granules, Golgi complex, endoplasmic reticulum Other Applications: Immunofluorescence, Immunoprecipitation, Western Blotting.

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human tonsil stained with Thrombospondin antibody