Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antikörper

Antigen: Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)

Synonyme: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B

Klonalität: Monoklonal (DCS-72-F6)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125394

Produktbeschreibung

Weitere Bezeichnung: p27

Immunogen: BALB/C mice were injected with mouse recombinant p27 protein.

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: DCS-72-F6

Spezifität: This antibody is specific to a protein of 27 kD known as p27Kip1 a cell cycle regulatory mitotic inhibitor. This antibody recognizes an epitope between amino acids 83-204 of p27. This antibody does not show any cross reactivity with other related mitotic inhibitors.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colan carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Krebs, Zellzyklus

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human colon carcinoma stained with p27Kip1 antibody