Cyclin E (CCNE) Antikörper

Antigen: Cyclin E (CCNE)

Synonyme: Cyce, Ccne, CYCLE, cyce, fa18e07, wu:fa18e07, BG:DS07108.3, CDI7, CYCE, Cyc E, CyclE, CyeE, D-CycE, DmCycE, DmcyclinE, br37, cyclinE, dm-cycE, fond, l(2)05206, l(2)35Dd, l(2)br37, l(2)k02514, l(2)k02602, l(2)k05007, l35Dd, DmelCG3938, CG3938, NV18076

Klonalität: Monoklonal (HE12)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125380

Produktbeschreibung

Weitere Bezeichnung: Cyclin E

Immunogen: Synthetic peptide corresponding to human cyclin E protein.

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: HE12

Spezifität: This antibody recognizes a doublet of 50 kD and a single band of 42 kD identified as cyclin E. Cyclin proteins are a family of related proteins, which control the progression of the eukaryotic cell cycle. Overexpression of cyclin E shortens the length of the G1 phase, accelerating progression of S phase. The activity of cyclin E is mediated through its activation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of tumor suppressor proteins such as p16.

Synonyme: Cyce, Ccne, CYCLE, cyce, fa18e07, wu:fa18e07, BG:DS07108.3, CDI7, CYCE, Cyc E, CyclE, CyeE, D-CycE, DmCycE, DmcyclinE, br37, cyclinE, dm-cycE, fond, l(2)05206, l(2)35Dd, l(2)br37, l(2)k02514, l(2)k02602, l(2)k05007, l35Dd, DmelCG3938, CG3938, NV18076

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Zellzyklus

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin E antibody