Ki-67 Antikörper
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| Klonalität |
Monoklonal (SP6) |
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Wirt
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Reaktivität
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Applikation
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| Produktnummer |
ABIN125288 |
| Menge |
6ml (1 to 4 ug/ml) (Varianten) |
| Preis |
190.00 $ Zzgl. Versandkosten $35.00
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Immunogen
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A synthetic peptide from C-terminus of human Ki-67.
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Format
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Supernatant (prediluted)
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Isotyp
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IgG (Passende Sekundärantikörper)
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Klon
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SP6
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Spezifität
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Ki-67 is a nuclear protein, which is expressed in the proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. This antibody crossreacts with Human and rat.
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Applikationshinweise
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear
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Konzentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Lagerung
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Forschungsgebiet
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Proliferationsmarker, Zellzyklus
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Beschränkungen
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Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
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Alternativen zu Antigen "Ki-67", Typ "Antikörper" finden
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Wirte
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Kaninchen (43), Maus (18)
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Reaktivitäten
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Human (57), Ratte (Rattus) (23), Maus (14), Kaninchen (6), Hund (5), Pferd (5), Schwein (5), Rind (Kuh) (3), Huhn (2)
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Applikationen
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Immunhistochemie (Paraffinschnitte) (IHC (p)) (31), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (20), Durchflusszytometrie (FACS) (16), Immunfluoreszenz (IF) (16), Immunhistochemie (IHC) (14), Western Blot (WB) (13), ELISA (7), Immunzytochemie (ICC) (2), Enzyme Immunoassay (EIA) (1), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (1)
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Konjugate
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2)
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Epitope
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C-Term (8)
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Alternativen 
