Vimentin (VIM) Antikörper
| Antigen |
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| Synonyme |
FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime |
| Klonalität |
Monoklonal (SP20) |
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Wirt
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Reaktivität
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Human (144), Ratte (Rattus) (66), Maus (60), Huhn (31), Rind (Kuh) (26), Schwein (23), Hund (9), Säugetiere (8), Affe (6), Ziege (5), Hamster (5), Amphibien (4), Potoroo (2), Fisch (1), Primate (1), Kaninchen (1), Xenopus laevis (1)
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Applikation
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Western Blot (WB) (90), Immunfluoreszenz (IF) (68), Immunhistochemie (IHC) (55), Durchflusszytometrie (FACS) (54), Immunhistochemie (Paraffinschnitte) (IHC (p)) (52), ELISA (39), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (26), Immunzytochemie (ICC) (13), Immunpräzipitation (IP) (11), Enzyme Immunoassay (EIA) (9), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Transfizierte Zellkultur (TCC) (2), Dot Blot (Dot) (1)
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| Produktnummer |
ABIN125282 |
| Menge |
500ul (100ug/ml approximately) (Varianten) |
| Preis |
240.00 $ Zzgl. Versandkosten $35.00
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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Vimentin
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Immunogen
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Recombinant human vimentin protein.
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Format
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Supernatant
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Isotyp
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IgG (Passende Sekundärantikörper)
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Klon
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SP20
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Spezifität
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This antibody recognizes vimentin. Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms. This antibody crossreacts with Human.
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Applikationshinweise
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. No pretreatment is required for formalin fixed paraffin tissue sections. Positive Control: Sarcomas. Cellular localization: Cytoplasmic
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Konzentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Lagerung
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Forschungsgebiet
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Zell-/Gewebemarker, Zytoskelett
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Beschränkungen
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Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
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Alternativen zu Antigen "Vimentin (VIM)", Typ "Antikörper" finden
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Wirte
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Kaninchen (88), Maus (64), Huhn (8), Schaf (3), Meerschweinchen (2), Ziege (1)
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Reaktivitäten
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Human (144), Ratte (Rattus) (66), Maus (60), Huhn (31), Rind (Kuh) (26), Schwein (23), Hund (9), Säugetiere (8), Affe (6), Ziege (5), Hamster (5), Amphibien (4), Potoroo (2), Fisch (1), Primate (1), Kaninchen (1), Xenopus laevis (1)
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Applikationen
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Western Blot (WB) (90), Immunfluoreszenz (IF) (68), Immunhistochemie (IHC) (55), Durchflusszytometrie (FACS) (54), Immunhistochemie (Paraffinschnitte) (IHC (p)) (52), ELISA (39), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (26), Immunzytochemie (ICC) (13), Immunpräzipitation (IP) (11), Enzyme Immunoassay (EIA) (9), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Transfizierte Zellkultur (TCC) (2), Dot Blot (Dot) (1)
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Konjugate
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FITC (9), Biotin (8), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
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Epitope
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pSer82 (20), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)
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Alternativen 
