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CD3 (CD3E) Antikörper

Antigen

CD3 (CD3E)

Synonyme T3E, TCRE, FLJ18683
Klonalität Monoklonal (SP7)
Wirt
Alternativen

Kaninchen

Reaktivität
Alternativen

Human

Applikation
Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p))
Produktnummer ABIN125268
Menge 1ml  (100ug/ml approximately)  (Varianten)
Preis 258.00 $   Zzgl. Versandkosten $35.00
Lieferung nach
Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung CD3
Immunogen A synthetic 13-mer peptide corresponding to aa 156-168 of the epsilon chain of human CD3 protein.
Format Supernatant
Isotyp IgG  (Passende Sekundärantikörper)
Klon SP7
Spezifität The CD3 antigen is present on early thymocytes and mature T cells and is generally regarded as a Pan T cell marker. This antibody will help detect CD3 expression in normal and neoplastic tissues. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues. This antibody cross reacts with Human, Baboon, Monkey, Horse, Dog, and Cat.

Anwendungen

Applikationshinweise Titer/Working Dilution: This antibody may be diluted to a titer of 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear
Konzentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Lagerung Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Forschungsgebiet CD Antigene
Beschränkungen Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Alternativen

Alternativen zu Antigen "CD3 (CD3E)", Typ "Antikörper" finden
Wirte Maus (303), Ratte (53), Kaninchen (48), Hamster (35)
Reaktivitäten Human (284), Maus (100), Ratte (Rattus) (43), Hund (30), Schwein (26), Huhn (18), Rind (Kuh) (17), Affe (15), Primate (10), Vogel (4), Katze (4), Hamster (3), Pferd (3), Primaten (3), Rhesusaffen (2), Kaninchen (1)
Applikationen Durchflusszytometrie (FACS) (347), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (86), Immunpräzipitation (IP) (61), Funktionale Studien (Func) (49), Immunhistochemie (Paraffinschnitte) (IHC (p)) (46), Western Blot (WB) (36), Immunfluoreszenz (IF) (31), ELISA (19), Immunhistochemie (IHC) (17), Blockierende Antikörper (Inhibition) (10), Immunzytochemie (ICC) (6), Enzyme Immunoassay (EIA) (3), Zytotoxizitätstest (CyTox) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Konjugate FITC (76), Biotin (46), PE (39), APC (24), RPE (10), SPRD (7), Cy5 (6), Alexa Fluor 488 (4), Cy5,PE (4), DY647 (3), PE,Cy5 (3), PE,Cy7 (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy7,PE (2), PE,Cy5.5 (2), PE,DY647 (2), PE-Dyomics 647 (2), PerCP (2), RPE,Cy5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), Pacific Blue (1), RBITC (1), RPE,Cy7 (1)
Epitope AA 156-168 (4), AA 196-206 (1), C-Term (1)