Cytokeratin 7 (KRT7) Antikörper

Antigen: Cytokeratin 7 (KRT7)

Synonyme: KRT7, CK7, K7, Krt2-7, MGC11625, D15Wsu77e, SCL, K2C7, MGC3625, MGC129731

Klonalität: Monoklonal (LDS-68)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125243

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 7

Immunogen: BALB/C mice were immunized with human bladder carcinoma cell line, RT4.

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: LDS-68

Spezifität: This antibody recognizes keratin polypeptide of 54 kD. This antibody reacts specifically with most simple epithelial cells but shows no reaction with epithelial cells in the intestine or stomach. Cholangiocellular carcinoma, ductal carcinoma of breast and transitional carcinoma of urinary bladder are positive but colon rectal adenocarcinoma and hepatocellular carcinoma does not show any staining.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

van Niekerk, Jap, Ramaekers et al.: "Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues." in: The Journal of pathology, Vol. 165, Issue 2, pp. 145-52, 1992 (PubMed).

Poels, Jap, Ramaekers et al.: "Characterization of a hormone-producing ovarian carcinoma cell line." in: Gynecologic oncology, Vol. 32, Issue 2, pp. 203-14, 1989 (PubMed).