Ki-67 Antikörper

Antigen: Ki-67

Klonalität: Monoklonal (PP-67)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125236

Produktbeschreibung

Immunogen: A HeLa cell nuclear preparation was used as immunogen.

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: PP-67

Spezifität: This antibody reacts with Ki-67 antigen associated with cell proliferation and found throughout the cell cycle (G1, S, G2, M) but absent in resting cells. Ki-67 reacts with the Ki-67 nuclear antigen (345 and 395 kDa, and several lower molecular weight proteins) in immunoblotting. The product shows an immunohistochemical staining pattern similar to that obtained with other anti-Ki-67/MIB-1 reagents. This antibody is useful in the study of cell proliferation in neoplastic cell populations and with stimulated lymphocytes. It may be used in establishing the growth fraction of neoplasms.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Proliferationsmarker, Zellzyklus

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Houmand, Abrahamsen, Tinggaard Pedersen: "Relevance of Ki-67 expression in the classification of non-Hodgkin's lymphomas: a morphometric and double-immunostaining study." in: Histopathology, Vol. 20, Issue 1, pp. 13-20, 1992 (PubMed).