MutS Homolog 6 (E. Coli) (MSH6) Antikörper

Antigen: MutS Homolog 6 (E. Coli) (MSH6)

Synonyme: GTBP, HSAP, HNPCC5, cb326, wu:fb36c12, MSH6, LOC100227810

Klonalität: Monoklonal (44)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Hund

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125226

Produktbeschreibung

Weitere Bezeichnung: MSH6

Immunogen: Synthetic human MSH6 peptide.

Format: Ig-fraction

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 44

Spezifität: MSH6 is a heterodimer of MSH2 and binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. This antibody cross reacts with mouse, rat and dog.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Tonsil, colon carcinoma. Cellular localization: Nuclear Other Applications: immunoprecipitation and Western blotting (1:100-500).

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as affinity purified immunoglobulin liquid containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human colon carcinoma stained with MSH6 antibody

Publikationen

Publikationen: Schleger, Heck, Niketeghad et al.: "Establishment and characterization of a nontumorigenic cell line derived from a human hepatocellular adenoma expressing hepatocyte-specific markers." in: Experimental cell research, Vol. 236, Issue 2, pp. 418-26, 1997 (PubMed).

Fasano, Theise, Nalesnik et al.: "Immunohistochemical evaluation of hepatoblastomas with use of the hepatocyte-specific marker, hepatocyte paraffin 1, and the polyclonal anti-carcinoembryonic antigen." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 11, Issue 10, pp. 934-8, 1998 (PubMed).

Bressenot, Marcon, Montagne et al.: "Clear cell adenocarcinoma: a rare variant of primary colonic tumour." in: International journal of colorectal disease, Vol. 23, Issue 1, pp. 137-8, 2007 (PubMed).