BRCA Antikörper

Antigen: BRCA

Klonalität: Monoklonal (GLK-2)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125222

Produktbeschreibung

Immunogen: Peptide corresponding to amino acids 1839-1863 of the c-terminus of BRCA1.

Format: Supernatant

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: GLK-2

Spezifität: BRCA1 recognizes a 22kD protein. This antibody stains 94% of ovarian carcinomas with a mutation in exon 11. However, staining was absent in 100% of tumors with mutations other than exon 11.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Ovarian carcinoma. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Kashima, Oite, Aoki et al.: "Screening of BRCA1 mutation using immunohistochemical staining with C-terminal and N-terminal antibodies in familial ovarian cancers." in: Japanese journal of cancer research : Gann, Vol. 91, Issue 4, pp. 399-409, 2000 (PubMed).