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CD3 (CD3E) Antikörper

Antigen

CD3 (CD3E)

Synonyme T3E, TCRE, FLJ18683
Klonalität Monoklonal (F7-2-38)
Wirt
Reaktivität
Alternativen

Human

Applikation
Alternativen Immunhistochemie (IHC)
Produktnummer ABIN125220
Menge 1ml  (100ug/ml approximately)  (Varianten)
Preis 526.00 $   Zzgl. Versandkosten $35.00
Lieferung nach
Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung CD3
Immunogen Purified CD3 epsilon gamma delta/CD3 omega
Format Supernatant
Isotyp IgG1  (Passende Sekundärantikörper)
Klon F7-2-38
Spezifität CD3 is first detectable in early Thymocytes and its appearance probably represents one of the earliest signs of commitment to T cell lineage. In cortical Thymocytes, the antigen is predominantly present as an intracytoplasmic constituent. This antibody labels CD3 epsilon and is useful tool for the identification of T cells and related neoplasms.

Anwendungen

Applikationshinweise Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 1 mM EDTA buffer, pH 9.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Membrane and cytoplasmic
Konzentration 100ug/ml approximately
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Lagerung Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Forschungsgebiet CD Antigene
Beschränkungen Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Alternativen

Alternativen zu Antigen "CD3 (CD3E)", Typ "Antikörper" finden
Wirte Maus (302), Ratte (53), Kaninchen (49), Hamster (35)
Reaktivitäten Human (284), Maus (100), Ratte (Rattus) (43), Hund (30), Schwein (26), Huhn (18), Rind (Kuh) (17), Affe (15), Primate (10), Vogel (4), Katze (4), Hamster (3), Pferd (3), Primaten (3), Rhesusaffen (2), Kaninchen (1)
Applikationen Durchflusszytometrie (FACS) (347), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (86), Immunpräzipitation (IP) (61), Funktionale Studien (Func) (49), Immunhistochemie (Paraffinschnitte) (IHC (p)) (47), Western Blot (WB) (36), Immunfluoreszenz (IF) (31), ELISA (19), Immunhistochemie (IHC) (16), Blockierende Antikörper (Inhibition) (10), Immunzytochemie (ICC) (6), Enzyme Immunoassay (EIA) (3), Zytotoxizitätstest (CyTox) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Konjugate FITC (76), Biotin (46), PE (39), APC (24), RPE (10), SPRD (7), Cy5 (6), Alexa Fluor 488 (4), Cy5,PE (4), DY647 (3), PE,Cy5 (3), PE,Cy7 (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy7,PE (2), PE,Cy5.5 (2), PE,DY647 (2), PE-Dyomics 647 (2), PerCP (2), RPE,Cy5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), Pacific Blue (1), RBITC (1), RPE,Cy7 (1)
Epitope AA 156-168 (4), AA 196-206 (1), C-Term (1)