Linker For Activation of T Cells (LAT) Antikörper

Antigen: Linker For Activation of T Cells (LAT)

Synonyme: LAT1, pp36, MGC108666, LAT, MGC157208, lat

Klonalität: Monoklonal (03-08-2007)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125212

Produktbeschreibung

Immunogen: Prokaryotic recombinant protein corresponding to a region of 120 amino acids with in the C-terminus of the LAT molecule.

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 03-08-2007

Spezifität: Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD), which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Membrane

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Immunologie

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Fukazawa, Reedquist, Panchamoorthy et al.: "T cell activation-dependent association between the p85 subunit of the phosphatidylinositol 3-kinase and Grb2/phospholipase C-gamma 1-binding phosphotyrosyl protein pp36/38." in: The Journal of biological chemistry, Vol. 270, Issue 34, pp. 20177-82, 1995 (PubMed).

Facchetti, Chan, Zhang et al.: "Linker for activation of T cells (LAT), a novel immunohistochemical marker for T cells, NK cells, mast cells, and megakaryocytes: evaluation in normal and pathological conditions." in: The American journal of pathology, Vol. 154, Issue 4, pp. 1037-46, 1999 (PubMed).