Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) Antikörper

Antigen: Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2)

Synonyme: COX2, COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, GRIPGHS, Cox-2, Pghs2, TIS10, Cox2, PTGS2, Ptgs2, cox-2, cox2, MGC80431, phs-2, hcox-2, pgg/hs, pghs-2, MGC97821, PGHS2

Klonalität: Monoklonal (4H12)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125211

Produktbeschreibung

Weitere Bezeichnung: Cyclooxygenase-2 (COX-2)

Immunogen: Prokaryotic recombinant protein corresponding to amino acids 30 to 163 of the N-terminus of the human COX-2 molecule.

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 4H12

Spezifität: COX-2 is a mitogen-inducible form of Cyclooxygenase, which is expressed in response to various inflammatory stimuli. COX-2 staining increases in the more differentiated, suprabasal keratinocytes of normal skin. COX-2 is expressed in epithelial and interstitial cells of adenomatous polyps and Colon adenocarcinomas.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Colon Ca. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Enzyme, Inflammation

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Isoherranen, Punnonen, Jansen et al.: "Ultraviolet irradiation induces cyclooxygenase-2 expression in keratinocytes." in: The British journal of dermatology, Vol. 140, Issue 6, pp. 1017-22, 2000 (PubMed).

Koemhoff, Guan, Shappell et al.: "Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas." in: The American journal of pathology, Vol. 157, Issue 1, pp. 29-35, 2000 (PubMed).

van Rees, Saukkonen, Ristimaeki et al.: "Cyclooxygenase-2 expression during carcinogenesis in the human stomach." in: The Journal of pathology, Vol. 196, Issue 2, pp. 171-9, 2002 (PubMed).