Retinoblastoma Gene Protein Antikörper

Antigen: Retinoblastoma Gene Protein

Klonalität: Monoklonal (1F8)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN125208

Produktbeschreibung

Weitere Bezeichnung: Retinoblastoma Gene Protein (Rb)

Immunogen: Recombinant human Rb protein.

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 1F8

Spezifität: This antibody recognizes human retinoblastoma gene protein. Rb gene, a prototype of tumor suppressor genes has been associated with development and/or progression of bladder cancer as well as sarcoma and small cell lung cancer. Functional Rb gene loss has been reported to occur in bladder cancer and osteosarcoma. A significantly poorer tumor-free survival rate was noted for those patients who had a tumor with an altered Rb protein with or without vascular invasion.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1mM EDTA buffer, pH 8.0 prior to immunostaining. Positive Control: Colan carcinoma. Cellular localization: Nuclear Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Krebs, Transkriptionsfaktoren

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human breast carcinoma stained with Retinoblastoma protein antibody

Publikationen

Publikationen: Weintraub, Prater, Dean: "Retinoblastoma protein switches the E2F site from positive to negative element." in: Nature, Vol. 358, Issue 6383, pp. 259-61, 1992 (PubMed).

Wang, To, Lee et al.: "Tumor suppressor activity of RB and p53 genes in human breast carcinoma cells." in: Oncogene, Vol. 8, Issue 2, pp. 279-88, 1993 (PubMed).

Nihei, Takahashi, Sagae et al.: "Protein interaction of retinoblastoma gene product pRb110 with M(r) 73,000 heat shock cognate protein." in: Cancer research, Vol. 53, Issue 7, pp. 1702-5, 1993 (PubMed).