Cytokeratin 8/18 Antikörper

Antigen: Cytokeratin 8/18

Klonalität: Monoklonal (5D3)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125207

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 8 & 18

Immunogen: Cytokeratins from the human breast carcinoma cell line MCF-7.

Format: Supernatant

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: 5D3

Spezifität: This antibody is specific to human cytokeratins 8 (52.5 kD) and 18 (45 kD). This antibody stains simple and glandular epithelium. This antibody can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Skin. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human mammacarcinoma stained with Cytokeratin 8/18 antibody