Carbohydrate Antigen 15-3 (CA15-3) Antikörper

Antigen: Carbohydrate Antigen 15-3 (CA15-3)

Synonyme: MUC1

Klonalität: Monoklonal (DF3)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN125179

Produktbeschreibung

Weitere Bezeichnung: CA 15-3

Immunogen: BALB/C mice were injected with membrane rich fraction of human metastatic breast carcinoma.

Format: Ascites

Isotyp: IgG1 kappa

Klon: DF3

Spezifität: This antibody is specific to 300 kD DF3 antigen present on the apical borders of secretory mammary epithelial cells. It stains glandular epithelia of breast, colon, kidney, liver, lung, pancreas, salivary gland and skin. In tumor tissues, it stains carcinomas of the breast, gastrointestinal tract, lung, kidney, urinary bladder and ovary.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic, cell membrane

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Krebs

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.